Inline Pathogen Detection - The Next Generation of Microbiological Testing
 
Early Warning’s Biohazard Water Analyzer is the world’s first inline diagnostic biosensor. Using a unique combination of advanced technologies, the Analyzer goes beyond lab culturing of indicator coliforms and directly measures individual species of pathogenic bacteria, protozoa and viruses in the same test. The Analyzer employs a revolutionary nanotechnology-based biosensor exclusively licensed from NASA and an on-board concentrator that processes a 10 liter water sample. The sample-to-report time is about 3 hours, and allows rapid prevention measures to be enacted. There is no need for time-intensive processes like transporting a water sample to the lab or Polymerase Chain Reaction (PCR). The Analyzer can be used as a transportable testing device or as a sensor node in a fully automated field sensor network. Users benefit from:

• Faster Time – Pathogen detection test results are transmitted electronically in about 3 hours after initiating a test
 
• Critical Information – Get the presence, concentration and viability of specific pathogens 

• Cost Effectiveness – No expensive laboratory or trained operators for sampling or testing are needed so water can be tested more frequency

• Reduce Risk – Automatically test to verify turbidity spikes, sudden drop in chlorine levels, drop in pressure, or other suspicious events
   
 
The Biohazard Water Analyzer goes beyond traditional testing by automatically sampling and measuring
actual pathogen concentration and viability in under 3 hours without a technician or biosafety laboratory
 
  
 
              Applications for Early Adopters

Water Utilities
• Rapid and remote field testing after a turbidity spike or suspicious event
• Contaminated source water downstream from farms, sewage treatment 
  plant outfalls, stormwater and combined sewage overflow discharges, 
  biotech industries, or wildlife
• Recycled water used for drinking
• Network infiltration from aging pipelines or sudden pressure drops
• Water security 

Food & Beverage
• Biosecurity and safe feed water for livestock, slaughterhouses,
  dairy products, and food and beverage plants
• Irrigation and crop processing employing contaminated source or 
  recycled water
• Continuous water monitoring for aquaculture, fisheries and marine 
  preservation
• Discharge from food processing facilities
• Automated sampling throughout the food production and distribution 
  system

Industrial Automation & Life Sciences
• Bacteria-sensitive products and media
• Control loop for sanitation equipment effectiveness such as 
  monitoring UV dose and energy use based on pathogen type before 
  UV and pathogen viability after UV
• Discharge from processing facilities
• Bacteria in cooling towers 

Point of Use (POU) Water Consumers
• Beach operators posting water quality updates onsite and online  
  within hours of sampling
• Hospitals and elderly care monitoring drinking and shower water to 
  immuno-compromised, sick and elderly
• Transportation vessels, cruise ships, and airlines collecting under-
  treated drinking water at foreign destinations
• Institutional water consumers with large populations such as hotels, 
  universities, multinationals, and government agencies
• Special protection for VIPs, celebrities, entertainers, diplomats and 
  luxury resorts

Biothreat & Homeland Security
• Inline biosensor in CBRN systems
• Remote monitoring of water utilities in violation of regulations
• Rapid inspection of imported fruits and vegetables at border crossings
  before entry into the country
• Disaster relief
• Military theaters and remote bases

Biotesting Services
• New laboratory and field services
 Biohazard Water Analyzer
Limited Release Beta Version
 
 
  
 
The Biohazard Water Analyzer employs a 5 stage process:

Filtration extracts bacteria, protozoa and viruses from a 10 Liter water sample by removing filtered water

Magnetic Bead Separation separates target pathogens from non-specific organisms and chemicals

Microfluidics extract RNA of target pathogens and permit the RNA to hybridize with matching DNA probes attached to specific working electrodes on a biochip

Electro-chemical Detection measures the generated current from guanine oxidation of individual target pathogens

Signal Processor calibrates the generated signals with known concentrations and transmits test results through wired or wireless communications
 
 
 
 
 



Last Updated:
25 Feb 2010

***** As many as 19 million Americans may become ill each year due to parasites, viruses and bacteria in drinking water
***** Cholera outbreak kills 4,037 and infects over 91,000 people from water in Zimbabwe
***** 40 dead, 2,000 infected as first cholera outbreak in 50 years spreads across Papua New Guinea
Rain forest atrium in senior citizens facility linked to 2 deaths from Legionnaires' disease in Vernon Hills, Il
City officials violate regulated levels of cancer-causing THM to disinfect drinking water from bacteria-infested biofilms in West Glouster, MA
Legionnaire's disease outbreak in water system at at the senior facility on the former site of Memorial Stadium in Baltimore, MD
Leptospirosis bacteria outbreak spread by water contaminated with the urine of rats, dogs and other animals after two tropical storms in the Philippines
***** H1N1 Swine flu pandemic kills over 5,000 and infects 414,000 in 122 countries
Typhoid outbreak from water in Taveuni, Fuji
Citizens Fear They Were Exposed to Contaminated Drinking Water for Months in Sugar Grove, Virginia
Crops, ponds destroyed in quest for food safety following E.coli O157:H7 outbreak in Salinas Valley, California
Diluted cleaning solution responsible for 1 death and died and 14 infected from C. difficile at Saanich Peninsula Hospital in British Columbia
E. Coli O157:H7 in Nestlι Toll House Refrigerated Cookie Dough infects 69 and hospitalizes 34 in US
10 die of hand foot and mouth disease in China
Woman dies of flesh eating infection in Pearl, Hawaii
Ticks carrying Lyme disease found in Niagara, Ontario
Possible E. coli contamination prompts the recall of 4,000 pounds of ground beef in New York
Students infected by salmonella from pudding in Madison, Wisconsin
Bacteria found in school’s water system in Parker, Indiana
Spring water source closed because of high Coliform bacteria levels in New York



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